Measuring ALL6 and ALL7 spacing
The spacings of ALL6 and ALL7 should be measured in the following region:
0.013 nm-1 < R < 0.053 nm-1
You need to calculate how many pixels the ROI to the meridian, namely X1 and X2
Assume: λ=0.1033 nm; 1 pixel=0.075mm; Sample to detector=1833mm, thus
1/0.013 nm =(0.1033nm∗1833mm)/(0.075mm/pixel∗X1)
X1=33 pixels
1/0.053 nm=(0.1033nm∗1833mm)/(0.075mm/pixel∗X2)
X2=134 pixels
So the ROI will be from: meridian +or- 33 pixel to meridian +or-134 pixel
### +or- depend on which side you are measuring ###
Where to cite
Wakabayashi, K., Sugimoto, Y., Tanaka, H., Ueno, Y., Takezawa, Y., & Amemiya, Y. (1994). X-ray diffraction evidence for the extensibility of actin and myosin filaments during muscle contraction. Biophysical journal, 67(6), 2422–2435. https://doi.org/10.1016/S0006-3495(94)80729-5
MuscleX Lab 